Wednesday, 14 October 2009

3rd year biochemistry practicals - Bradford assay

Hey ya all,

I know you sometimes have a problems with the calculations (do not worry me too, but we will handle anything). This week we have been using the Bradford assay to estimate protein concentration of your last week samples.

All the necessary calculations, plots and tables you need to put in your lab report are here:

Page 1

Page 2

Page 3

One more thing. Chose the most accurate standard curve and use that for the calculations. Remember to comment on your choice.

Remember to include the dilution factor of your samples, what I mean is that if you are looking at the 1/10 dilution of your sample you have to multiply your result by then to obtain your initial conncentration and so on.

To calculate the total enzyme activity you have to multiply the Normal Enzyme Avctivity by the Volume of the enzyme you had (remember to include the loss of it during the centrifugation, if it happend to you).

Have fun. CyA next week. 


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